产品名称
CLASP1 Rabbit Polyclonal Antibody
别名
CLASP1; KIAA0622; MAST1; CLIP-associating protein 1; Cytoplasmic linker-associated protein 1; Multiple asters homolog 1; Protein Orbit homolog 1; hOrbit1
蛋白名称
CLIP-associating protein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23332
Human Swissprot No.
Q7Z460
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q7Z460/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=76707
Mouse Swissprot No.
Q80TV8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q80TV8
免疫原
The antiserum was produced against synthesized peptide derived from human CLASP1. AA range:1171-1220
特异性
CLASP1 Polyclonal Antibody detects endogenous levels of CLASP1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
cytoplasmic linker associated protein 1(CLASP1) Homo sapiens CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008],
组织表达
Brain,Endometrium carcinoma cell line,Epithelium,Eye,Platelet,Poole
细胞定位
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus, trans-Golgi network . Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.
信号通路
Regulation of Microtubule Dynamics
功能
function:Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the CLASP family.,similarity:Contains 7 HEAT repeats.,subcellular location:Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.,subunit:Interacts with CLIP2, ERC1, MAPRE1, MAPRE3, microtubules, PHLDB2 and RSN. The interaction with ERC1 may be mediated by PHLDB2.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
