BD-PT0912-CHOP Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CHOP Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0912

产品名称

CHOP Rabbit Polyclonal Antibody

别名

DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; Growth arrest and DNA damage-inducible protein GADD153

类别

常规抗体

基因名称

DDIT3

蛋白名称

DNA damage-inducible transcript 3 protein

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1649

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1649

Human Swissprot No.

P35638

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P35638/entry

Mouse Gene ID

13198

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13198

Mouse Swissprot No.

P35639

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P35639

免疫原

The antiserum was produced against synthesized peptide derived from human CHOP. AA range:15-64

特异性

CHOP Polyclonal Antibody detects endogenous levels of CHOP protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

19kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010],

组织表达

Muscle,Skeletal muscle,

细胞定位

Cytoplasm . Nucleus . Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation. .

信号通路

MAPK_ERK_Growth;MAPK_G_Protein;

功能

disease:A chromosomal aberration involving DDIT3 is found in a form of malignant myxoid liposarcoma [MIM:126337]. Translocation t(12;16)(q13;p11) with FUS.,function:Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,subunit:Heterodimer.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using CHOP Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using CHOP Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa, Jurkat, and COLO205 cells, using CHOP Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using CHOP Polyclonal Antibody diluted at 1：500

Western Blot analysis of VEC cells using CHOP Polyclonal Antibody diluted at 1：500

Western blot analysis of various lysis using CHOP Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000