BD-PT0877

c-FLIP Rabbit Polyclonal Antibody

CFLAR; CASH; CASP8AP1; CLARP; MRIT; CASP8 and FADD-like apoptosis regulator; Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FAD

常规抗体

CFLAR

CASP8 and FADD-like apoptosis regulator

WB

Human,Rat,Mouse

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

8837

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8837

O15519

http://www.uniprot.org/uniprotkb/O15519/entry

O35732

http://www.uniprot.org/uniprot/O35732

The antiserum was produced against synthesized peptide derived from human in ternal CFLAR. AA range:181-230

c-FLIP Polyclonal Antibody detects endogenous levels of c-FLIP protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

55kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011],

Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.

cytoplasm,cytosol,death-inducing signaling complex,CD95 death-inducing signaling complex,membrane raft,ripoptosome,

Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;

domain:The caspase domain lacks the active sites residues involved in catalysis.,function:Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.,induction:Repressed by IL-2 after TCR stimulation, during progression to the S-phase of the cell cycle.,PTM:Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 2 DED (death effector) domains.,subunit:TNFRSF6 stimulation triggers recruitment to the death-inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X.,tissue specificity:Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.