产品名称
PLCD4 Rabbit Polyclonal Antibody
别名
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 (hPLCD4) (EC 3.1.4.11) (Phosphoinositide phospholipase C-delta-4) (Phospholipase C-delta-4) (PLC-delta-4)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.299% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/84812
Human Swissprot No.
Q9BRC7
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9BRC7/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/18802
Mouse Swissprot No.
Q8K3R3
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8K3R3
Rat Gene Link
https://www.uniprot.org/uniprot/140693
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q62711
免疫原
Synthesized peptide derived from human PLCD4 AA range: 82-132
特异性
This antibody detects endogenous levels of PLCD4 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. [provided by RefSeq, Jan 2011],
组织表达
Highly expressed in skeletal muscle and kidney tissues, and at moderate level in intestinal tissue. Expressed in corneal epithelial cells.
细胞定位
Membrane ; Peripheral membrane protein . Nucleus . Cytoplasm . Endoplasmic reticulum . Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.
功能
catalytic activity:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.,cofactor:Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain.,domain:The C2 domain mediates pre-localization to the membrane prior to Ca(2+) import and non-selective Ca(2+)-mediated targeting to various cellular membranes.,domain:The PDZ-binding motif mediates the interaction with GRIP1.,domain:The PH domain is not a critical determinant of the membrane localization.,function:Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression upregulates the Erk signaling pathway and proliferation.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 PI-PLC X-box domain.,similarity:Contains 1 PI-PLC Y-box domain.,similarity:Contains 3 EF-hand domains.,subcellular location:Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.,subunit:Interacts with GRIP1.,tissue specificity:Highly expressed in skeletal muscle and kidney tissues, and at moderate level in intestinal tissue. Expressed in corneal epithelial cells.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
