BD-PT0856-CEP135 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CEP135 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0856

产品名称

CEP135 Rabbit Polyclonal Antibody

别名

CEP135; CEP4; KIAA0635; Centrosomal protein of 135 kDa; Cep135; Centrosomal protein 4

类别

常规抗体

基因名称

CEP135

蛋白名称

Centrosomal protein of 135 kDa

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

9662

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9662

Human Swissprot No.

Q66GS9

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q66GS9/entry

Mouse Gene ID

381644

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=381644

Mouse Swissprot No.

Q6P5D4

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q6P5D4

免疫原

The antiserum was produced against synthesized peptide derived from human CEP135. AA range:1081-1130

特异性

CEP135 Polyclonal Antibody detects endogenous levels of CEP135 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

预测分子量

134kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

centrosomal protein 135(CEP135) Homo sapiens This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8. [provided by RefSeq, Jun 2012],

组织表达

Brain,Epithelium,Placenta,

细胞定位

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.

功能

similarity:Belongs to the CEP135/TSGA10 family.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from COLO cells, using CEP135 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of COLO cells using CEP135 Polyclonal Antibody diluted at 1：1000

Western blot analysis of mouse-lung mouse-heart 293T lysis using CEP135 antibody. Antibody was diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.