产品名称
Cdc42EP5 Rabbit Polyclonal Antibody
别名
CDC42EP5; BORG3; CEP5; Cdc42 effector protein 5; Binder of Rho GTPases 3
蛋白名称
Cdc42 effector protein 5
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=148170
Human Swissprot No.
Q6NZY7
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q6NZY7/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=58804
Mouse Swissprot No.
Q9Z0X0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9Z0X0
免疫原
The antiserum was produced against synthesized peptide derived from human BORG3. AA range:1-50
特异性
Cdc42EP5 Polyclonal Antibody detects endogenous levels of Cdc42EP5 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Cell division control protein 42 (CDC42), a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg (binder of Rho GTPases) family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to CDC42 and regulate its function negatively. The encoded protein may inhibit c-Jun N-terminal kinase (JNK) independently of CDC42 binding. The protein may also play a role in septin organization and inducing pseudopodia formation in fibroblasts [provided by RefSeq, Jul 2013],
细胞定位
Endomembrane system ; Peripheral membrane protein . Cytoplasm, cytoskeleton .
功能
domain:The CRIB domain mediates interaction with CDC42.,function:Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Inhibits MAPK8 independently of CDC42 binding. Controls septin organization and this effect is negatively regulated by CDC42.,similarity:Belongs to the BORG/CEP family.,similarity:Contains 1 CRIB domain.,subunit:Interacts with CDC42, in a GTP-dependent manner, and with SEPT7.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
