产品名称
Cdc27 Rabbit Polyclonal Antibody
别名
CDC27; ANAPC3; D0S1430E; D17S978E; Cell division cycle protein 27 homolog; Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC
蛋白名称
Cell division cycle protein 27 homolog
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=996
Human Swissprot No.
P30260
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P30260/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=217232
Mouse Swissprot No.
A2A6Q5
Mouse Swissprot Link
http://www.uniprot.org/uniprot/A2A6Q5
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=360643
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q4V8A2
免疫原
The antiserum was produced against synthesized peptide derived from human H-NUC. AA range:361-410
特异性
Cdc27 Polyclonal Antibody detects endogenous levels of Cdc27 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been i
细胞定位
Nucleus . Cytoplasm, cytoskeleton, spindle .
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Oocyte meiosis;Ubiquitin mediated proteolysis;Progesterone-mediated oocyte maturation;
功能
PTM:Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.,similarity:Belongs to the APC3/CDC27 family.,similarity:Contains 9 TPR repeats.,subunit:Interacts with RB.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
