产品名称
Cdc25B Rabbit Polyclonal Antibody
别名
CDC25B; CDC25HU2; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B
蛋白名称
M-phase inducer phosphatase 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=994
Human Swissprot No.
P30305
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P30305/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12531
Mouse Swissprot No.
P30306
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P30306
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=171103
Rat Swissprot Link
http://www.uniprot.org/uniprot/P48966
免疫原
The antiserum was produced against synthesized peptide derived from human CDC25B. AA range:289-338
特异性
Cdc25B Polyclonal Antibody detects endogenous levels of Cdc25B protein.
稀释度
WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
cell division cycle 25B(CDC25B) Homo sapiens CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Progesterone-mediated oocyte maturation;
功能
catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,enzyme regulation:Stimulated by B-type cyclins.,function:Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.,PTM:Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein.,similarity:Belongs to the MPI phosphatase family.,similarity:Contains 1 rhodanese domain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
