BD-PT0796

Cdc25A Rabbit Polyclonal Antibody

CDC25A; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A

常规抗体

CDC25A

M-phase inducer phosphatase 1

WB

Human,Mouse,Rat,Monkey

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

993

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=993

P30304

http://www.uniprot.org/uniprotkb/P30304/entry

P48964

http://www.uniprot.org/uniprot/P48964

171102

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=171102

P48965

http://www.uniprot.org/uniprot/P48965

The antiserum was produced against synthesized peptide derived from human CDC25A. AA range:43-92

Cdc25A Polyclonal Antibody detects endogenous levels of Cdc25A protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

60kD

-20°C/1 year

Polyclonal, Rabbit,IgG

cell division cycle 25A(CDC25A) Homo sapiens CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Lymph,

intracellular,nucleus,nucleoplasm,cytoplasm,cytosol,

Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Progesterone-mediated oocyte maturation;

catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,domain:The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.,enzyme regulation:Stimulated by B-type cyclins.,function:Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.,PTM:Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A.,PTM:Ubiquitinated. Association with the F-box proteins BTRC and FBXW11 targets the protein for ubiquitination by CUL1 and proteolysis by the ubiquitin-dependent proteasome pathway.,similarity:Belongs to the MPI phosphatase family.,similarity:Contains 1 rhodanese domain.,subunit:Interacts with CCNB1/cyclin B1. Interacts with YWHAE/14-3-3 epsilon when phosphorylated. Interacts with CUL1 specifically when CUL1 is neddylated and active. Interacts with BTRC/BTRCP1 and FBXW11/BTRCP2. Interactions with CUL1, BTRC and FBXW11 are enhanced upon DNA damage.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.