产品名称
Cdc16 Rabbit Polyclonal Antibody
别名
CDC16; ANAPC6; Cell division cycle protein 16 homolog; Anaphase-promoting complex subunit 6; APC6; CDC16 homolog; CDC16Hs; Cyclosome subunit 6
蛋白名称
Cell division cycle protein 16 homolog
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8881
Human Swissprot No.
Q13042
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13042/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=69957
Mouse Swissprot No.
Q8R349
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8R349
免疫原
The antiserum was produced against synthesized peptide derived from human APC6. AA range:181-230
特异性
Cdc16 Polyclonal Antibody detects endogenous levels of Cdc16 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. [provided by RefSeq, Jan 2016],
组织表达
Aorta endothelial cell,Brain,Epithelium,Lung,Skin,
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Oocyte meiosis;Ubiquitin mediated proteolysis;Progesterone-mediated oocyte maturation;
功能
function:Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.,similarity:Belongs to the APC6/CDC16 family.,similarity:Contains 7 TPR repeats.,subcellular location:Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.,subunit:The APC/C is composed of at least 11 subunits. Interacts with PPP5C and CDC20.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
