产品名称
Cdc14a phosphatase Rabbit Polyclonal Antibody
别名
CDC14A; Dual specificity protein phosphatase CDC14A; CDC14 cell division cycle 14 homolog A
蛋白名称
Dual specificity protein phosphatase CDC14A
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8556
Human Swissprot No.
Q9UNH5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9UNH5/entry
Mouse Swissprot No.
Q6GQT0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q6GQT0
免疫原
Synthesized peptide derived from the Internal region of human Cdc14a phosphatase.
特异性
Cdc14a phosphatase Polyclonal Antibody detects endogenous levels of Cdc14a phosphatase protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
cell division cycle 14A(CDC14A) Homo sapiens The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],
组织表达
Aorta endothelial cell,Brain,Placenta,
细胞定位
Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, spindle . Cell projection, kinocilium . Cell projection, stereocilium . Centrosomal during interphase, released into the cytoplasm at the onset of mitosis. Subsequently localizes to the mitotic spindle pole and at the central spindle (PubMed:12134069, PubMed:11901424, PubMed:15263015). Present along both the transient kinocilia of developing cochlear hair cells and the persistent kinocilia of vestibular hair cells (By similarity). .
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;
功能
catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,domain:Composed of two structurally equivalent A and B domains that adopt a dual specificity protein phosphatase (DSP) fold.,function:Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC-FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.,subcellular location:Centrosomal during interphase, released into the cytoplasm at the onset of mitosis. Subsequently localizes to the midzone of the mitotic spindle.,subunit:Interacts with KIF20A, which is required to localize CDC14 to the midzone of the mitotic spindle.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
