BD-PT0776-CD75 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD75 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0776

产品名称

CD75 Rabbit Polyclonal Antibody

别名

ST6GAL1; SIAT1; Beta-galactoside alpha-2; 6-sialyltransferase 1; Alpha 2,6-ST 1; B-cell antigen CD75; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; ST6Gal I; ST6GalI; Sialyltransferase 1

类别

常规抗体

基因名称

ST6GAL1

蛋白名称

Beta-galactoside alpha-2,6-sialyltransferase 1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

6480

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6480

Human Swissprot No.

P15907

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P15907/entry

Mouse Gene ID

20440

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20440

Mouse Swissprot No.

Q64685

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q64685

Rat Gene ID

25197

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25197

Rat Swissprot No.

P13721

Rat Swissprot Link

http://www.uniprot.org/uniprot/P13721

免疫原

The antiserum was produced against synthesized peptide derived from human ST6GAL1. AA range:171-220

特异性

CD75 Polyclonal Antibody detects endogenous levels of CD75 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

预测分子量

42kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq, Aug 2009],

组织表达

Liver,Lymph,Placenta,Skin,Spleen,Thymus,

细胞定位

Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein . Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.

信号通路

N-Glycan biosynthesis;

功能

catalytic activity:CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.,function:Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.,online information:GlycoGene database,online information:ST6Gal I,pathway:Protein modification; protein glycosylation.,PTM:The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.,PTM:The soluble form derives from the membrane form by proteolytic processing.,similarity:Belongs to the glycosyltransferase 29 family.,subcellular location:Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using ST6GAL1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from RAW264.7 cells, using ST6GAL1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of COLO cells using CD75 Polyclonal Antibody diluted at 1：2000

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.