BD-PT0757-CD34 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD34 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0757

产品名称

CD34 Rabbit Polyclonal Antibody

别名

CD34; Hematopoietic progenitor cell antigen CD34; CD antigen CD34

类别

常规抗体

基因名称

CD34

蛋白名称

Hematopoietic progenitor cell antigen CD34

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

947

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=947

Human Swissprot No.

P28906

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P28906/entry

Mouse Gene ID

12490

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12490

Mouse Swissprot No.

Q64314

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q64314

免疫原

The antiserum was produced against synthesized peptide derived from human CD34. AA range:208-257

特异性

CD34 Polyclonal Antibody detects endogenous levels of CD34 protein.

稀释度

WB 1:500-2000;IHC-p 1:100-500;IF ICC 1:100-500;ELISA 1:5000-20000

预测分子量

110kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],

组织表达

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

细胞定位

Membrane; Single-pass type I membrane protein.

信号通路

Cell adhesion molecules (CAMs);Hematopoietic cell lineage;

功能

alternative products:Both isoforms are expressed on the cell surface. CD34-T/CD34-F ratio increases with cell differentiation,developmental stage:On early hematopoietic progenitor cells.,disease:Abnormal CD34 expression in leukemogenesis.,function:Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.,online information:CD34 entry,PTM:Highly glycosylated.,PTM:Phosphorylated on serine residues by PKC.,similarity:Belongs to the CD34 family.,tissue specificity:Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from HepG2 cells, using CD34 antibody.

Western Blot analysis of various cells using CD34 Polyclonal Antibody diluted at 1：2000

Western Blot analysis of HepG2 cells using CD34 Polyclonal Antibody diluted at 1：2000

mouse-lung mouse-brain mouse-spleen mouse-kidney

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.