BD-PT0752-CD31 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD31 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0752

产品名称

CD31 Rabbit Polyclonal Antibody

别名

PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31

类别

常规抗体

基因名称

PECAM1

蛋白名称

Platelet endothelial cell adhesion molecule

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

5175

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5175

Human Swissprot No.

P16284

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P16284/entry

Mouse Gene ID

18613

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18613

Mouse Swissprot No.

Q08481

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q08481

免疫原

The antiserum was produced against synthesized peptide derived from human PECAM-1. AA range:686-735

特异性

CD31 Polyclonal Antibody detects endogenous levels of CD31 protein.

稀释度

WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

预测分子量

85-130kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010],

组织表达

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580308). Expressed on neutrophils (at protein level) (PubMed:17580308). Isoform Long predominates in all tissues examined (PubMed:12433657). Isoform Delta12 is detected only in trachea (PubMed:12433657). Isoform Delta14-15 is only detected in lung (PubMed:12433657). Isoform Delta14 is detected in all tissues examined with the strongest expression in heart (PubMed:12433657). Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) (PubMed:12433657, PubMed:18388311).

细胞定位

Cell membrane ; Single-pass type I membrane protein . Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8-mediated stimulation. .; [Isoform Long]: Cell membrane ; Single-pass type I membrane protein . Membrane raft . Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. .; [Isoform Delta15]: Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

信号通路

Cell adhesion molecules (CAMs);Leukocyte transendothelial migration;

功能

function:This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.,online information:CD31 entry,online information:PECAM-1,online information:The Singapore human mutation and polymorphism database,PTM:Phosphorylated on Ser and Tyr residues after cellular activation.,similarity:Contains 6 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using PECAM-1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PECAM-1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using PECAM-1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HT-29 cells using PECAM-1 antibody.

Western Blot analysis of various cells using CD31 Polyclonal Antibody diluted at 1：1000

Western Blot analysis of rat-musle cells using CD31 Polyclonal Antibody diluted at 1：1000

Western blot analysis of KB lysis using CD31 antibody. Antibody was diluted at 1:1000

Western blot analysis of 293T lysis using CD31 antibody. Antibody was diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:400（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:400（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.