产品名称
CB1 Rabbit Polyclonal Antibody
别名
CNR1; CNR; Cannabinoid receptor 1; CB-R; CB1; CANN6
蛋白名称
Cannabinoid receptor 1
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1268
Human Swissprot No.
P21554
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P21554/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12801
Mouse Swissprot No.
P47746
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P47746
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25248
Rat Swissprot Link
http://www.uniprot.org/uniprot/P20272
免疫原
The antiserum was produced against synthesized peptide derived from human CNR1. AA range:151-200
特异性
CB1 Polyclonal Antibody detects endogenous levels of CB1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009],
组织表达
Widely expressed, with highest levels in fetal and adult brain. Expression levels of isoform 2 and isoform 3 are much lower than those of isoform 1.
细胞定位
Cell membrane ; Multi-pass membrane protein . Membrane raft . Mitochondrion outer membrane . Cell projection, axon . Cell junction, synapse, presynapse . Unexpectedly, in the mitochondria, the C-terminus is located in the mitochondrial intermembrane space, a compartment topologically considered as extracellular. In canonical seven-transmembrane G-protein coupled receptors, the C-terminus is cytosolic (By similarity). Found on presynaptic axon terminals in some GABAergic neurons in the somatosensory cortex (By similarity). .
信号通路
Neuroactive ligand-receptor interaction;
功能
function:Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.,similarity:Belongs to the G-protein coupled receptor 1 family.,subunit:Interacts (via C-terminus) with CNRIP1.,tissue specificity:Widely expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
