BD-PT0686-Caveolin-1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Caveolin-1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0686

产品名称

Caveolin-1 Rabbit Polyclonal Antibody

别名

CAV1; CAV; Caveolin-1

类别

常规抗体

基因名称

CAV1

蛋白名称

Caveolin-1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

857

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=857

Human Swissprot No.

Q03135

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q03135/entry

Mouse Gene ID

12389

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12389

Mouse Swissprot No.

P49817

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P49817

Rat Swissprot No.

P41350

Rat Swissprot Link

http://www.uniprot.org/uniprot/P41350

免疫原

The antiserum was produced against synthesized peptide derived from human Caveolin-1. AA range:129-178

特异性

Caveolin-1 Polyclonal Antibody detects endogenous levels of Caveolin-1 protein.

稀释度

WB 1:500-2000, IF 1:50-300, IHC 1:50-300

预测分子量

25kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010],

组织表达

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

细胞定位

Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola ; Peripheral membrane protein. Membrane raft . Golgi apparatus, trans-Golgi network . Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.

信号通路

Focal adhesion;Viral myocarditis;

功能

disease:Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.,function:May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity.,online information:Caveolin entry,PTM:The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.,similarity:Belongs to the caveolin family.,subcellular location:Potential hairpin-like structure in the membrane. Membrane protein of caveolae.,subunit:Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4.,tissue specificity:In muscle and lung, less so in liver, brain and kidney.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HUVEC cells, using Caveolin-1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HUVEC cells, using Caveolin-1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of human-lung tissue. 1,Caveolin-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-lung tissue. 1,Caveolin-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-kidney tissue. 1,Caveolin-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of various cells using primary antibody diluted at 1:1000（4°C overnight）. Secondary antibody：Goat Anti-rabbit IgG IRDye 800（ diluted at 1:5000, 25°C, 1 hour）. Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit（SM-005, Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Caveolin-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Caveolin-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,Caveolin-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,Caveolin-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using Caveolin-1 Polyclonal Antibody diluted at 1：1000

Western Blot analysis of HuvEc cells using Caveolin-1 Polyclonal Antibody diluted at 1：1000

Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.