产品名称
Cathepsin?G Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1511
Human Swissprot No.
P08311
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P08311/entry
Mouse Swissprot No.
P28293
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P28293
免疫原
The antiserum was produced against synthesized peptide derived from human Cathepsin?G. AA range:67-116
特异性
Cathepsin?G Polyclonal Antibody detects endogenous levels of Cathepsin?G protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014],
组织表达
Expressed in neutrophils (at protein level) (PubMed:3799965). Expressed in B cells (PubMed:15100291).
细胞定位
Cell membrane ; Peripheral membrane protein . Cytoplasmic granule . Secreted . Cytoplasm, cytosol . Lysosome . Nucleus . Secreted by activated neutrophils (PubMed:3390156). Detected in synovial fluid (PubMed:32144329). Localizes to lysosomes in B cells where it is not endogenously synthesized but is internalized from the cell membrane (PubMed:15100291). Localizes to the nucleus during apoptosis (PubMed:11259672). .
信号通路
Neuroactive ligand-receptor interaction;Lysosome;Renin-angiotensin system;Systemic lupus erythematosus;
功能
catalytic activity:Specificity similar to chymotrypsin C.,enzyme regulation:Inhibited by soybean trypsin inhibitor, benzamidine, the synthetic peptide R13K, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. Inhibited by LPS from P.aeruginosa but not by LPS from S.minnesota.,function:Serine protease with trypsin- and chymotrypsin-like specificity. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 peptidase S1 domain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
