产品名称
Cathepsin?E Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1510
Human Swissprot No.
P14091
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P14091/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13034
Mouse Swissprot No.
P70269
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P70269
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25424
Rat Swissprot Link
http://www.uniprot.org/uniprot/P16228
免疫原
The antiserum was produced against synthesized peptide derived from human Cathepsin E. AA range:243-292
特异性
Cathepsin?E Polyclonal Antibody detects endogenous levels of Cathepsin?E protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration. [provided by RefSeq, Nov 2015],
组织表达
Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.
细胞定位
Endosome . The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.
功能
catalytic activity:Similar to cathepsin D, but slightly broader specificity.,function:May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.,PTM:Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide.,PTM:Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.,similarity:Belongs to the peptidase A1 family.,subcellular location:The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.,subunit:Homodimer; disulfide-linked.,tissue specificity:Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
