BD-PT0653

Caspase-10 B/C Rabbit Polyclonal Antibody

ALPS2; apoptotic protease Mch-4; CASP-10; CASPA; caspase-10; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-convert

常规抗体

CASP10

Caspase10

WB

Human,Rat,Mouse

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

843

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=843

Q92851-2/4

http://www.uniprot.org/uniprotkb/Q92851-2/entry

The antiserum was produced against synthesized peptide derived from human Caspase 10. AA range:430-479

Caspase-10 B/D Polyclonal Antibody detects endogenous levels of proCaspase-10 B/D protein, actived Caspase-10 (isoform B and D) and Caspase-10(isoform B and D) subunit p12,

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

pro: 60kD, actived: 33kD 12kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011],

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

cytosol,CD95 death-inducing signaling complex,ripoptosome,

catalytic activity:Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Leu-Gln-Thr-Asp-|-Gly.,disease:Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.,disease:Defects in CASP10 are a cause of gastric cancers [MIM:137215].,disease:Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.,function:Isoform C is proteolytically inactive.,online information:CASP10 mutation db,online information:Caspase-10 mutations causing ALPS type II,PTM:Cleavage by granzyme B and autocatalytic activity generate the two active subunits.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 2 DED (death effector) domains.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit (By similarity). Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10.,tissue specificity:Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.