产品名称
Casein Kinase Iε Rabbit Polyclonal Antibody
别名
CSNK1E; Casein kinase I isoform epsilon; CKI-epsilon; CKIe
蛋白名称
Casein kinase I isoform epsilon
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1454
Human Swissprot No.
P49674
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P49674/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=27373
Mouse Swissprot No.
Q9JMK2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JMK2
免疫原
The antiserum was produced against synthesized peptide derived from human CKI-epsilon. AA range:276-325
特异性
Casein Kinase Iε Polyclonal Antibody detects endogenous levels of Casein Kinase Iε protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
casein kinase 1 epsilon(CSNK1E) Homo sapiens The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014],
组织表达
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
细胞定位
Cytoplasm . Nucleus .
信号通路
WNT;WNT-T CELLHedgehog;Circadian rhythm;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.,induction:Down-regulated during granulocytic differentiation.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with ANKRD6 and SOCS3.,tissue specificity:Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
