产品名称
CARD 10 Rabbit Polyclonal Antibody
别名
CARD10; CARMA3; Caspase recruitment domain-containing protein 10; CARD-containing MAGUK protein 3; Carma 3
蛋白名称
Caspase recruitment domain-containing protein 10
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29775
Human Swissprot No.
Q9BWT7
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9BWT7/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=105844
Mouse Swissprot No.
P58660
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P58660
免疫原
The antiserum was produced against synthesized peptide derived from human CARD10. AA range:481-530
特异性
CARD 10 Polyclonal Antibody detects endogenous levels of CARD 10 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008],
组织表达
Detected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney.
功能
caution:Supposed to contain a SH3, a PDZ and a guanylate kinase-like domain. But none of these 3 domains are detected by PROSITE, Pfam or SMART.,function:Activates NF-kappa-B via BCL10 and IKK.,similarity:Contains 1 CARD domain.,subunit:CARD10 and BCL10 bind to each other by CARD-CARD interaction. They both participate in a complex with MALT1, where MALT1 binds to BCL10.,tissue specificity:Detected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
