BD-PT0620

Calregulin Rabbit Polyclonal Antibody

CALR; CRTC; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60

常规抗体

CALR

Calreticulin

WB

Human,Mouse,Rat,Monkey

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

811

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=811

P27797

http://www.uniprot.org/uniprotkb/P27797/entry

12317

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12317

P14211

http://www.uniprot.org/uniprot/P14211

64202

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64202

P18418

http://www.uniprot.org/uniprot/P18418

The antiserum was produced against synthesized peptide derived from human CALR. AA range:21-70

Calregulin Polyclonal Antibody detects endogenous levels of Calregulin protein.

WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;IF ICC 1:100-500;ELISA 1:5000-20000

48kD

-20°C/1 year

Polyclonal, Rabbit,IgG

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its

Brain,Cajal-Retzius cell,Colon carcinoma,Eye,Fetal brain cortex,Keratinocyte,Liver,Pancreas

Endoplasmic reticulum lumen . Cytoplasm, cytosol . Secreted, extracellular space, extracellular matrix . Cell surface . Sarcoplasmic reticulum lumen . Cytoplasmic vesicle, secretory vesicle, Cortical granule . Cytolytic granule . Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). During oocyte maturation and after parthenogenetic activation accumulates in cortical granules. In pronuclear and early cleaved embryos localizes weakly to cytoplasm around nucleus and more strongly in the region near the cortex (By similarity). In cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation (By similarity). .

Antigen processing and presentation;

caution:Was originally (PubMed:2332496) thought to be the 52 kDa Ro autoantigen.,domain:Associates with PDIA3 through the tip of the extended arm formed by the P-domain.,domain:Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.,domain:The interaction with glycans occurs through a binding site in the globular lectin domain.,domain:The zinc binding sites are localized to the N-domain.,function:Molecular calcium binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.,mass spectrometry: PubMed:11149926,online information:Calreticulin,online information:Calreticulin entry,similarity:Belongs to the calreticulin family.,subcellular location:Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.,subunit:Monomer. Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 and with NR3C1.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.