BD-PT0612-Calmodulin Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Calmodulin Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0612

产品名称

Calmodulin Rabbit Polyclonal Antibody

别名

CALM1; CALM; CAM; CAM1; CALM2; CAM2; CAMB; CALM3; CALML2; CAM3; CAMC; CAMIII; Calmodulin; CaM

类别

常规抗体

基因名称

CALM1

蛋白名称

Calmodulin

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

801/805/808

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=801

Human Swissprot No.

P62158

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P62158/entry

Mouse Gene ID

12313

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12313

Mouse Swissprot No.

P62204

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P62204

Rat Gene ID

24242

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24242

Rat Swissprot No.

P62161

Rat Swissprot Link

http://www.uniprot.org/uniprot/P62161

免疫原

The antiserum was produced against synthesized peptide derived from human Calmodulin. AA range:46-95

特异性

Calmodulin Polyclonal Antibody detects endogenous levels of Calmodulin protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

参考分子量

17kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009],

组织表达

Blood,Brain,Cajal-Retzius cell,Fetal brain cortex,Lung,Lymph,Lymphoma,Muscle,Osteosarcoma,P

细胞定位

spindle pole,extracellular region,nucleus,nucleoplasm,cytoplasm,centrosome,cytosol,spindle microtubule,plasma membrane,voltage-gated potassium channel complex,sarcomere,growth cone,vesicle,calcium channel complex,G

信号通路

Calcium;Phosphatidylinositol signaling system;Oocyte meiosis;Vascular smooth muscle contraction;Long-term potentiation;Neurotrophin;Olfactory transduction;Insulin_Receptor;GnRH;Melanogenesis;Alzheimer's disease;Glioma;

功能

function:Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.,miscellaneous:This protein has four functional calcium-binding sites.,PTM:Phosphorylation results in a decreased activity.,PTM:Ubiquitination results in a strongly decreased activity.,similarity:Belongs to the calmodulin family.,similarity:Contains 4 EF-hand domains.,subcellular location:Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.,subunit:Interacts with MYO1C (By similarity). Interacts with CEP97, CEP110, TTN/titin and SRY.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HepG2 cells, using Calmodulin Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Calmodulin Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, using Calmodulin Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Calmodulin Polyclonal Antibody diluted at 1：2000

Western Blot analysis of HuvEc cells using Calmodulin Polyclonal Antibody diluted at 1：2000

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.