产品名称
CA XII Rabbit Polyclonal Antibody
别名
CA12; Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3
蛋白名称
Carbonic anhydrase 12
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=771
Human Swissprot No.
O43570
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43570/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=76459
Mouse Swissprot No.
Q8CI85
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8CI85
免疫原
The antiserum was produced against synthesized peptide derived from human CA12. AA range:231-280
特异性
CA XII Polyclonal Antibody detects endogenous levels of CA XII protein.
稀释度
IHC-p: 100-300.WB 1:500 - 1:2000. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014],
组织表达
Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677).
细胞定位
Membrane; Single-pass type I membrane protein. Cell membrane .
信号通路
Nitrogen metabolism;
功能
catalytic activity:H(2)CO(3) = CO(2) + H(2)O.,cofactor:Zinc.,enzyme regulation:Inhibited by acetazolamide.,function:Reversible hydration of carbon dioxide.,similarity:Belongs to the alpha-carbonic anhydrase family.,tissue specificity:Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
