产品名称
CA IX Rabbit Polyclonal Antibody
别名
CA9; G250; MN; Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1
蛋白名称
Carbonic anhydrase 9
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=768
Human Swissprot No.
Q16790
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q16790/entry
Mouse Swissprot No.
Q8VHB5
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8VHB5
免疫原
The antiserum was produced against synthesized peptide derived from human CA IX. AA range:33-82
特异性
CA IX Polyclonal Antibody detects endogenous levels of CA IX protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014],
组织表达
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
细胞定位
Nucleus . Nucleus, nucleolus . Cell membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein . Found on the surface microvilli and in the nucleus, particularly in nucleolus.
信号通路
Nitrogen metabolism;
功能
catalytic activity:H(2)CO(3) = CO(2) + H(2)O.,cofactor:Zinc.,function:Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.,induction:By hypoxia.,PTM:Asn-346 bears high-mannose type glycan structures.,similarity:Belongs to the alpha-carbonic anhydrase family.,subcellular location:Found on the surface microvilli and in the nucleus, particularly in nucleolus.,subunit:Forms oligomers linked by disulfide bonds.,tissue specificity:Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
