产品名称
CA II Rabbit Polyclonal Antibody
别名
CA2; Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II
蛋白名称
Carbonic anhydrase 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=760
Human Swissprot No.
P00918
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P00918/entry
Mouse Swissprot No.
P00920
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P00920
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54231
Rat Swissprot Link
http://www.uniprot.org/uniprot/P27139
免疫原
The antiserum was produced against synthesized peptide derived from human CA II. AA range:180-229
特异性
CA II Polyclonal Antibody detects endogenous levels of CA II protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014],
细胞定位
Cytoplasm . Cell membrane . Colocalized with SLC26A6 at the surface of the cell membrane in order to form a bicarbonate transport metabolon. Displaced from the cytosolic surface of the cell membrane by PKC in phorbol myristate acetate (PMA)-induced cells. .
信号通路
Nitrogen metabolism;
功能
catalytic activity:H(2)CO(3) = CO(2) + H(2)O.,cofactor:Zinc.,disease:Defects in CA2 are the cause of autosomal recessive osteopetrosis type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.,function:Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide.,similarity:Belongs to the alpha-carbonic anhydrase family.,subunit:Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
