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C/EBP ζ Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT0559
产品名称
C/EBP ζ Rabbit Polyclonal Antibody
别名
CEBPZ; CBF2; CCAAT/enhancer-binding protein zeta; CCAAT-box-binding transcription factor; CBF; CCAAT-binding factor
类别
常规抗体
基因名称
CEBPZ
蛋白名称
CCAAT/enhancer-binding protein zeta
推荐应用
WB
反应种属
Human,Rat,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
10153
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10153
Human Swissprot No.
Q03701
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q03701/entry
Mouse Swissprot No.
P53569
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P53569
免疫原
The antiserum was produced against synthesized peptide derived from human CEBPZ. AA range:1-50
特异性
C/EBP ζ Polyclonal Antibody detects endogenous levels of C/EBP ζ protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
预测分子量
120kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene belongs to the CEBP family. The encoded protein plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner. The protein is also involved in cell growth and differentiation, particularly, hematopoietic differentiation. Methylation of the promoter of this gene or mutations within the gene may be correlated with occurance of acute myeloid leukemia (AML). [provided by RefSeq, Jun 2016],
组织表达
Epithelium,Testis,
细胞定位
Nucleus.
功能
function:Stimulates transcription from the HSP70 promoter.,similarity:Belongs to the CBF/MAK21 family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from COLO cells, using CEBPZ Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using C/EBP ζ Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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