BD-PT0558-C/EBP ε Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

C/EBP ε Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0558

产品名称

C/EBP ε Rabbit Polyclonal Antibody

别名

CEBPE; CCAAT/enhancer-binding protein epsilon; C/EBP epsilon

类别

常规抗体

基因名称

CEBPE

蛋白名称

CCAAT/enhancer-binding protein epsilon

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1053

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1053

Human Swissprot No.

Q15744

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q15744/entry

Mouse Swissprot No.

Q6PZD9

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q6PZD9

免疫原

The antiserum was produced against synthesized peptide derived from human C/EBP-epsilon. AA range:40-89

特异性

C/EBP ε Polyclonal Antibody detects endogenous levels of C/EBP ε protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

34kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008],

组织表达

Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.

细胞定位

Nucleus .

功能

function:C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.,online information:CEBPE mutation db,PTM:Phosphorylated.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP delta.,tissue specificity:Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using C/EBP-epsilon Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using C/EBP-epsilon Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from JurKat cells, treated with Insulin 0.01U/ml 15‘, using C/EBP-epsilon Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using C/EBP ε Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of Jurkat cells using C/EBP ε Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.