BD-PT0555-C/EBP γ Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

C/EBP γ Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0555

产品名称

C/EBP γ Rabbit Polyclonal Antibody

别名

CEBPG; CCAAT/enhancer-binding protein gamma; C/EBP gamma

类别

常规抗体

基因名称

CEBPG

蛋白名称

CCAAT/enhancer-binding protein gamma

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1054

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1054

Human Swissprot No.

P53567

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P53567/entry

Mouse Gene ID

12611

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12611

Mouse Swissprot No.

P53568

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P53568

Rat Gene ID

25301

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25301

Rat Swissprot No.

P26801

Rat Swissprot Link

http://www.uniprot.org/uniprot/P26801

免疫原

The antiserum was produced against synthesized peptide derived from human CEBPG. AA range:31-80

特异性

C/EBP γ Polyclonal Antibody detects endogenous levels of C/EBP γ protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

22kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2011],

组织表达

Placenta,T-cell,Trachea,

细胞定位

Nucleus .

功能

function:Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha and beta and with Fos protein.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CEBPG Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from RAW264.7 cells, using CEBPG Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of RAW264.7 cells using C/EBP γ Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.