产品名称
BUB1 Rabbit Polyclonal Antibody
别名
BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A
蛋白名称
Mitotic checkpoint serine/threonine-protein kinase BUB1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=699
Human Swissprot No.
O43683
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43683/entry
Mouse Swissprot No.
O08901
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O08901
免疫原
The antiserum was produced against synthesized peptide derived from human BUB1. AA range:781-830
特异性
BUB1 Polyclonal Antibody detects endogenous levels of BUB1 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
组织表达
High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
细胞定位
Nucleus. Chromosome, centromere, kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, KNL1 and INCENP are required for kinetochore localization (By similarity). .
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Oocyte meiosis;Progesterone-mediated oocyte maturation;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in BUB1 are associated with tumor formation.,domain:CD1 domain directs kinetochore localization and binding to BUB3.,enzyme regulation:Autophosphorylated when the cells enters mitosis.,function:Involved in cell cycle checkpoint enforcement. Can interact and phosphorylate BUB3.,induction:Inhibited by phorbol 12-myristate 13-acetate (PMA).,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.,similarity:Contains 1 CD1 domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Nuclear in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage.,tissue specificity:High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
