BD-PT0495-BM28 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BM28 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0495

产品名称

BM28 Rabbit Polyclonal Antibody

别名

MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

类别

常规抗体

基因名称

MCM2

蛋白名称

DNA replication licensing factor MCM2

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4171

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4171

Human Swissprot No.

P49736

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P49736/entry

Mouse Gene ID

17216

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17216

Mouse Swissprot No.

P97310

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P97310

免疫原

The antiserum was produced against synthesized peptide derived from human MCM2. AA range:1-50

特异性

BM28 Polyclonal Antibody detects endogenous levels of BM28 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

120kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012],

组织表达

Bone marrow,Brain,Cervix carcinoma,Colon carcinoma,Epitheli

细胞定位

Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .

信号通路

DNA replication;Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;

功能

function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.,PTM:Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex.,sequence caution:Translation N-terminally shortened.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,subunit:Interacts with DBF4 (By similarity). Interacts with MYST2. May interact with MCM10.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HepG2 cells, using MCM2 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MCM2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using MCM2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using BM28 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of 293 cells using BM28 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.