产品名称
BIG2 Rabbit Polyclonal Antibody
别名
ARFGEF2; ARFGEP2; BIG2; Brefeldin A-inhibited guanine nucleotide-exchange protein 2; Brefeldin A-inhibited GEP 2; ADP-ribosylation factor guanine nucleotide-exchange factor 2
蛋白名称
Brefeldin A-inhibited guanine nucleotide-exchange protein 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10564
Human Swissprot No.
Q9Y6D5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9Y6D5/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=99371
Mouse Swissprot No.
A2A5R2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/A2A5R2
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=296380
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q7TSU1
免疫原
The antiserum was produced against synthesized peptide derived from human ARFGEF2. AA range:1491-1540
特异性
BIG2 Polyclonal Antibody detects endogenous levels of BIG2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008],
组织表达
Expressed in placenta, lung, heart, brain, kidney and pancreas.
细胞定位
Cytoplasm. Membrane. Golgi apparatus. Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network . Endosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, dendrite . Cytoplasmic vesicle . Cell junction, synapse . Cytoplasm, cytoskeleton . Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
功能
disease:Defects in ARFGEF2 are the cause of autosomal recessive periventricular nodular heterotopia type 2 (PVNH2) [MIM:608097]; also called periventricular heterotopia with microcephaly autosomal recessive. PVNH2 is an autosomal recessive form characterized by microcephaly (small brain), severe developmental delay and recurrent infections. No anomalies extrinsic to the central nervous system, such as dysmorphic features or grossly abnormal endocrine or other conditions, are associated with PVNH2.,enzyme regulation:Inhibited by brefeldin A.,function:Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 SEC7 domain.,tissue specificity:Expressed in placenta, lung, heart, brain, kidney and pancreas.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
