BD-PT0488

BID Rabbit Polyclonal Antibody

BID; BH3-interacting domain death agonist; p22 BID; BID

常规抗体

BID

BH3-interacting domain death agonist

WB

Human,Rat,Mouse

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

637

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=637

P55957

http://www.uniprot.org/uniprotkb/P55957/entry

12122

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12122

P70444

http://www.uniprot.org/uniprot/P70444

The antiserum was produced against synthesized peptide derived from human BID. AA range:44-93

BID Polyclonal Antibody detects endogenous levels of BID protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

25kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008],

[Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level). ; [Isoform 3]: Expressed in lung, pancreas and spleen (at protein level). ; [Isoform 4]: Expressed in lung and pancreas (at protein level).

Cytoplasm . Mitochondrion membrane . Mitochondrion outer membrane . When uncleaved, it is predominantly cytoplasmic. .; [BH3-interacting domain death agonist p15]: Mitochondrion membrane . Translocates to mitochondria as an integral membrane protein. .; [BH3-interacting domain death agonist p13]: Mitochondrion membrane . Associated with the mitochondrial membrane. .; [Isoform 1]: Cytoplasm .; [Isoform 3]: Cytoplasm .; [Isoform 2]: Mitochondrion membrane . A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. .

p53;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Natural killer cell mediated cytotoxicity;Alzheimer's disease;Amyotrophic lateral sclerosis (ALS);Pathways in cancer;Viral myocarditis;

domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.,subcellular location:A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.,subcellular location:Associated with the mitochondrial membrane.,subcellular location:Translocates to mitochondria as an integral membrane protein.,subcellular location:When uncleaved, it is predominantly cytoplasmic.,subunit:Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2.,tissue specificity:Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.