产品名称
BCKDK Rabbit Polyclonal Antibody
别名
BCKDK; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN
蛋白名称
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10295
Human Swissprot No.
O14874
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O14874/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12041
Mouse Swissprot No.
O55028
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O55028
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29603
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q00972
免疫原
The antiserum was produced against synthesized peptide derived from human BCKD . AA range:11-60
特异性
BCKDK Polyclonal Antibody detects endogenous levels of BCKDK protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012],
细胞定位
Mitochondrion matrix. Mitochondrion .
功能
catalytic activity:ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate.,function:Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.,PTM:Autophosphorylated.,similarity:Belongs to the PDK/BCKDK protein kinase family.,similarity:Contains 1 histidine kinase domain.,subunit:Monomer.,tissue specificity:Ubiquitous.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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