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ATP5L2 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT0409
产品名称
ATP5L2 Rabbit Polyclonal Antibody
别名
ATP5L2; ATP5K2; ATP synthase subunit g 2; mitochondrial; ATPase subunit g 2
类别
常规抗体
基因名称
ATP5L2
蛋白名称
ATP synthase subunit g 2 mitochondrial
推荐应用
WB
反应种属
Human,Rat,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
267020
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=267020
Human Swissprot No.
Q7Z4Y8
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q7Z4Y8/entry
免疫原
The antiserum was produced against synthesized peptide derived from human ATP5L2. AA range:51-100
特异性
ATP5L2 Polyclonal Antibody detects endogenous levels of ATP5L2 protein.
稀释度
WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
预测分子量
20kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.,similarity:Belongs to the ATPase g subunit family.,subunit:F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L).,
组织表达
Liver,
细胞定位
Mitochondrion membrane .
功能
function:Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.,similarity:Belongs to the ATPase g subunit family.,subunit:F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L).,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using ATP5L2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using ATP5L2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HeLa cells using ATP5L2 antibody.

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