产品名称
ATP5H Rabbit Polyclonal Antibody
别名
ATP5H; My032; ATP synthase subunit d; mitochondrial; ATPase subunit d
蛋白名称
ATP synthase subunit d mitochondrial
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10476
Human Swissprot No.
O75947
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O75947/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=71679
Mouse Swissprot No.
Q9DCX2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9DCX2
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=641434
Rat Swissprot Link
http://www.uniprot.org/uniprot/P31399
免疫原
The antiserum was produced against synthesized peptide derived from human ATP5H. AA range:111-160
特异性
ATP5H Polyclonal Antibody detects endogenous levels of ATP5H protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010],
组织表达
Bone marrow,Brain,Fetal brain,Fetal
细胞定位
Mitochondrion. Mitochondrion inner membrane.
信号通路
Oxidative phosphorylation;Alzheimer's disease;Parkinson's disease;Huntington's disease;
功能
function:Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.,similarity:Belongs to the ATPase d subunit family.,subunit:F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
