产品名称
Atg4D Rabbit Polyclonal Antibody
别名
ATG4D; APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D
蛋白名称
Cysteine protease ATG4D
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84971
Human Swissprot No.
Q86TL0
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q86TL0/entry
Mouse Swissprot No.
Q8BGV9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BGV9
免疫原
Synthesized peptide derived from the Internal region of human Atg4D.
特异性
Atg4D Polyclonal Antibody detects endogenous levels of Atg4D protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
组织表达
Widely expressed in testis.
细胞定位
[Cysteine protease ATG4D]: Cytoplasm .; [Cysteine protease ATG4D, mitochondrial]: Cytoplasm . Mitochondrion matrix . Imported into mitochondrial matrix after cleavage by CASP3 during oxidative stress and cell death. .
信号通路
Regulation of autophagy;
功能
enzyme regulation:Inhibited by N-ethylmaleimide.,function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Mainly expressed in skeletal muscle and, to a lower extent, in testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
