产品名称
Atg4b Rabbit Polyclonal Antibody
别名
ATG4B; APG4B; AUTL1; KIAA0943; Cysteine protease ATG4B; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B
蛋白名称
Cysteine protease ATG4B
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23192
Human Swissprot No.
Q9Y4P1
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9Y4P1/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=66615
Mouse Swissprot No.
Q8BGE6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BGE6
免疫原
The antiserum was produced against synthesized peptide derived from human ATG4B. AA range:71-120
特异性
Atg4b Polyclonal Antibody detects endogenous levels of Atg4b protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
组织表达
Brain,Embryo,Endometrium,Epithelium,Hippocampus,Liver,Placenta,Test
细胞定位
Cytoplasm . Cytoplasm, cytosol . Cytoplasmic vesicle, autophagosome . Endoplasmic reticulum . Mitochondrion . Mainly localizes to the cytoplasm, including cytosol (PubMed:29165041). A samll potion localizes to mitochondria; phosphorylation at Ser-34 promotes localization to mitochondria (PubMed:29165041). .
信号通路
Regulation of autophagy;
功能
enzyme regulation:Inhibited by N-ethylmaleimide.,function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
