产品名称
Atg4A Rabbit Polyclonal Antibody
别名
ATG4A; APG4A; AUTL2; Cysteine protease ATG4A; AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A
蛋白名称
Cysteine protease ATG4A
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=115201
Human Swissprot No.
Q8WYN0
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8WYN0/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=666468
Mouse Swissprot No.
Q8C9S8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8C9S8
免疫原
The antiserum was produced against synthesized peptide derived from human ATG4A. AA range:81-130
特异性
Atg4A Polyclonal Antibody detects endogenous levels of Atg4A protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Mar 2016],
组织表达
Epithelium,Kidney,Ovary,Prostate,Testis,
信号通路
Regulation of autophagy;
功能
enzyme regulation:Inhibited by N-ethylmaleimide.,function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
