产品名称
ASC-1 Rabbit Polyclonal Antibody
别名
TRIP4; Activating signal cointegrator 1; ASC-1; Thyroid receptor-interacting protein 4; TR-interacting protein 4; TRIP-4
蛋白名称
Activating signal cointegrator 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9325
Human Swissprot No.
Q15650
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q15650/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56404
Mouse Swissprot No.
Q9QXN3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9QXN3
免疫原
The antiserum was produced against synthesized peptide derived from human TRIP4. AA range:81-130
特异性
ASC-1 Polyclonal Antibody detects endogenous levels of ASC-1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a subunit of the tetrameric nuclear activating signal cointegrator 1 (ASC-1) complex, which associates with transcriptional coactivators, nuclear receptors and basal transcription factors to facilitate nuclear receptors-mediated transcription. This protein is localized in the nucleus and contains an E1A-type zinc finger domain, which mediates interaction with transcriptional coactivators and ligand-bound nuclear receptors, such as thyroid hormone receptor and retinoid X receptor alpha, but not glucocorticoid receptor. Mutations in this gene are associated with spinal muscular atrophy with congenital bone fractures-1 (SMABF1). [provided by RefSeq, Apr 2016],
细胞定位
Nucleus . Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasmic under conditions of serum deprivation (PubMed:10454579). Colocalizes with NEK6 in the centrosome (PubMed:20873783). .
功能
function:Transcription coactivator of nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. Plays a role in androgen receptor transactivation and in testicular function.,subcellular location:Cytoplasmic under conditions of serum deprivation.,subunit:Specifically interacts with the ligand binding domain of the thyroid receptor (TR). This interaction requires the presence of thyroid hormone. Exists as a steady-state complex associated with ASCC1, ASCC2 and HELIC1. Interacts with the androgen receptor androgen (AR) in an androgen, testosterone and dihydrotestosterone-dependent manner.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
