产品名称
AR-β2 Rabbit Polyclonal Antibody
别名
ADRB2; ADRB2R; B2AR; Beta-2 adrenergic receptor; Beta-2 adrenoreceptor; Beta-2 adrenoceptor
蛋白名称
Beta-2 adrenergic receptor
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=154
Human Swissprot No.
P07550
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P07550/entry
Mouse Swissprot No.
P18762
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P18762
免疫原
The antiserum was produced against synthesized peptide derived from human Adrenergic Receptor beta2. AA range:321-370
特异性
AR-β2 Polyclonal Antibody detects endogenous levels of AR-β2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:5000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],
组织表达
Blood,Brain,Fetal brain,Heart,Leukocyte,Prostate,Thyroid,
细胞定位
Cell membrane ; Multi-pass membrane protein . Early endosome . Golgi apparatus . Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325). Activated receptors are also detected within the Golgi apparatus (PubMed:27481942). .
信号通路
Calcium;Neuroactive ligand-receptor interaction;Endocytosis;
功能
disease:Polymorphic forms of ADRB2 could impart some form of nocturnal asthma.,function:Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.,PTM:Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.,PTM:Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,subunit:Binds SLC9A3R1 and GPRASP1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
