产品名称
Arkadia Rabbit Polyclonal Antibody
别名
RNF111; E3 ubiquitin-protein ligase Arkadia; RING finger protein 111
蛋白名称
E3 ubiquitin-protein ligase Arkadia
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54778
Human Swissprot No.
Q6ZNA4
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q6ZNA4/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=93836
Mouse Swissprot No.
Q99ML9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q99ML9
免疫原
The antiserum was produced against synthesized peptide derived from human RNF111. AA range:901-950
特异性
Arkadia Polyclonal Antibody detects endogenous levels of Arkadia protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
ring finger protein 111(RNF111) Homo sapiens The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012],
细胞定位
Nucleus . Cytoplasm . Nucleus, PML body . Upon TGF-beta treatment, translocates from nucleus to cytosol. .
功能
function:Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Upon TGF-beta treatment, translocates from nucleus to cytosol.,subunit:Interacts with SMAD6, SMAD7, AXIN1, AXIN2 and SKIL isoform SNON. Part of a complex containing RNF111, AXIN1 and SMAD7.,tissue specificity:Broadly expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
