产品名称
ARHGAP22 Rabbit Polyclonal Antibody
别名
ARHGAP22; RHOGAP2; Rho GTPase-activating protein 22; Rho-type GTPase-activating protein 22
蛋白名称
Rho GTPase-activating protein 22
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=58504
Human Swissprot No.
Q7Z5H3
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q7Z5H3/entry
Mouse Swissprot No.
Q8BL80
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BL80
免疫原
The antiserum was produced against synthesized peptide derived from human RHG22. AA range:565-614
特异性
ARHGAP22 Polyclonal Antibody detects endogenous levels of ARHGAP22 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],
组织表达
Brain,Erythroleukemia,Lung,
细胞定位
Cytoplasm . Nucleus . Mainly cytoplasmic. Some fraction is nuclear (By similarity). .
功能
function:Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.,similarity:Contains 1 PH domain.,similarity:Contains 1 Rho-GAP domain.,subcellular location:Mainly cytoplasmic. Some fraction is nuclear.,subunit:Interacts with VEZF1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
