产品名称
ARHGAP17 Rabbit Polyclonal Antibody
别名
ARHGAP17; RICH1; MSTP066; MSTP110; Rho GTPase-activating protein 17; Rho-type GTPase-activating protein 17; RhoGAP interacting with CIP4 homologs protein 1; RICH-1
蛋白名称
Rho GTPase-activating protein 17
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55114
Human Swissprot No.
Q68EM7
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q68EM7/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=70497
Mouse Swissprot No.
Q3UIA2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q3UIA2
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=63994
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q99N37
免疫原
The antiserum was produced against synthesized peptide derived from human RHG17. AA range:331-380
特异性
ARHGAP17 Polyclonal Antibody detects endogenous levels of ARHGAP17 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA (MIM 165390), RAC1 (MIM 602048), and CDC42 (MIM 116952).[supplied by OMIM, Apr 2004],
组织表达
Ubiquitously expressed. Expressed at higher level in heart and placenta.
细胞定位
Membrane; Peripheral membrane protein. Cytoplasm. Cell junction, tight junction. Associates with membranes and concentrates at sites of cell-cell contact.
功能
domain:The BAR domain mediates the interaction with the coiled coil domain of AMOT, leading to its recruitment to tight junctions.,function:Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activitor in vitro for RAC1.,similarity:Contains 1 BAR domain.,similarity:Contains 1 Rho-GAP domain.,subcellular location:Associates with membranes and concentrates at sites of cell-cell contact.,subunit:Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Interacts with SLC9A3R1, FNBP1, TRIP10, CAPZA (CAPZA1, CAPZA2 or CAPZA3), CAPZB, CD2AP and SH3KBP1/CIN85.,tissue specificity:Ubiquitously expressed. Expressed at higher level in heart and placenta.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
