产品名称
Arginase I Rabbit Polyclonal Antibody
别名
ARG1; Arginase-1; Liver-type arginase; Type I arginase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=383
Human Swissprot No.
P05089
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P05089/entry
Mouse Swissprot No.
Q61176
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61176
免疫原
The antiserum was produced against synthesized peptide derived from human ARG1. AA range:61-110
特异性
Arginase I Polyclonal Antibody detects endogenous levels of Arginase I protein.
稀释度
IHC-p: 100-300.WB 1:500 - 1:2000. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
组织表达
Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]) (PubMed:15546957). Also detected in macrophages mycobacterial granulomas (PubMed:23749634). Expressed in group2 innate lymphoid cells (ILC2s) during lung disease (PubMed:27043409).
细胞定位
Cytoplasm . Cytoplasmic granule . Localized in azurophil granules of neutrophils (PubMed:15546957). .
信号通路
Arginine and proline metabolism;
功能
catalytic activity:L-arginine + H(2)O = L-ornithine + urea.,cofactor:Binds 2 manganese ions per subunit.,disease:Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.,induction:By arginine or homoarginine.,online information:Arginase entry,pathway:Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.,similarity:Belongs to the arginase family.,subunit:Homotrimer.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
