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Arfaptin 1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT0310
产品名称
Arfaptin 1 Rabbit Polyclonal Antibody
别名
ARFIP1; Arfaptin-1; ADP-ribosylation factor-interacting protein 1
类别
常规抗体
基因名称
ARFIP1
蛋白名称
Arfaptin-1
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
27236
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=27236
Human Swissprot No.
P53367
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P53367/entry
Rat Gene ID
60382
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=60382
Rat Swissprot No.
Q9JHU5
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q9JHU5
免疫原
The antiserum was produced against synthesized peptide derived from human ARFIP1. AA range:271-320
特异性
Arfaptin 1 Polyclonal Antibody detects endogenous levels of Arfaptin 1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
预测分子量
40kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Putative target protein of ADP-ribosylation factor.,similarity:Contains 1 AH domain.,subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.,tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.,
组织表达
Ubiquitously expressed (PubMed:9038142). Higher levels in liver, pancreas, placenta, skeletal muscle and heart (PubMed:9038142).
细胞定位
Golgi apparatus . Golgi apparatus, trans-Golgi network membrane .
功能
function:Putative target protein of ADP-ribosylation factor.,similarity:Contains 1 AH domain.,subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.,tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using ARFIP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using ARFIP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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