BD-PT0301

ARA54 Rabbit Polyclonal Antibody

RNF14; ARA54; HRIHFB2038; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein

常规抗体

RNF14

E3 ubiquitin-protein ligase RNF14

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

9604

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9604

Q9UBS8

http://www.uniprot.org/uniprotkb/Q9UBS8/entry

56736

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56736

Q9JI90

http://www.uniprot.org/uniprot/Q9JI90

The antiserum was produced against synthesized peptide derived from human RNF14. AA range:361-410

ARA54 Polyclonal Antibody detects endogenous levels of ARA54 protein.

WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

50kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011],

Widely expressed.

Cytoplasm . Nucleus .

caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,domain:The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.,domain:The RING-type zinc finger is essential for the interaction with UBE2E2.,function:Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.,pathway:Protein modification; protein ubiquitination.,PTM:RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.,similarity:Belongs to the RBR family. RNF14 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 1 RWD domain.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2 and in the presence of testosterone, with the androgen receptor (AR).,tissue specificity:Widely expressed.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.