产品名称
APS Rabbit Polyclonal Antibody
别名
SH2B2; APS; SH2B adapter protein 2; Adapter protein with pleckstrin homology and Src homology 2 domains; SH2 and PH domain-containing adapter protein APS
蛋白名称
SH2B adapter protein 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10603
Human Swissprot No.
O14492
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O14492/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23921
Mouse Swissprot No.
Q9JID9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JID9
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=114203
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q9Z200
免疫原
Synthesized peptide derived from the Internal region of human APS.
特异性
APS Polyclonal Antibody detects endogenous levels of APS protein.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is expressed in B lymphocytes and contains pleckstrin homology and src homology 2 (SH2) domains. In Burkitt's lymphoma cell lines, it is tyrosine-phosphorylated in response to B cell receptor stimulation. Because it binds Shc independent of stimulation and Grb2 after stimulation, it appears to play a role in signal transduction from the receptor to the Shc/Grb2 pathway. [provided by RefSeq, Jun 2009],
组织表达
Expressed in spleen, prostate, testis, uterus, small intestine and skeletal muscle. Among hematopoietic cell lines, expressed exclusively in B-cells. Not expressed in most tumor cell lines.
细胞定位
Cytoplasm . Cell membrane . Cytoplasmic before PDGF stimulation. After PDGF stimulation, localized at the cell membrane and peripheral region.
信号通路
Neurotrophin;Insulin_Receptor;
功能
function:Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways. May be involved in coupling from immunoreceptor to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis. May induce cytoskeletal reorganization via interaction with VAV3.,PTM:Tyrosine phosphorylated by JAK2, KIT and other kinases activated by B-cell receptor in response to stimulation with cytokines, IL3, IL5, PDGF, IGF1, IGF2, CSF2/GM-CSF and cross-linking of the B-cell receptor complex.,similarity:Belongs to the SH2B adapter family.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH2 domain.,subcellular location:Cytoplasmic before PDGF stimulation. After PDGF stimulation, localized at the cell membrane and peripheral region.,subunit:Interacts with several proteins including KIT/c-KIT, SHC, GRB2, EPOR, CBL/c-CBL, PDGFR, VAV1 and VAV3. Interacts with SHC through its N-terminal region, with GRB2 via the phosphorylated C-terminus and with EPOR and KIT via its SH2 domain. Interacts with GRB2 after B-cell antigen receptor stimulation. Interacts with VAV3 via its PH domain.,tissue specificity:Expressed in spleen, prostrate, testis, uterus, small intestine and skeletal muscle. Among haematopoietic cell lines, expressed exclusively in B-cells. Not expressed in most tumor cell lines.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
