BD-PT0254-AP-2γ Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AP-2γ Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0254

产品名称

AP-2γ Rabbit Polyclonal Antibody

别名

TFAP2C; Transcription factor AP-2 gamma; AP2-gamma; Activating enhancer-binding protein 2 gamma; Transcription factor ERF-1

类别

常规抗体

基因名称

TFAP2C

蛋白名称

Transcription factor AP-2 gamma

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7022

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7022

Human Swissprot No.

Q92754

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q92754/entry

Mouse Gene ID

21420

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=21420

Mouse Swissprot No.

Q61312

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q61312

免疫原

The antiserum was produced against synthesized peptide derived from human AP2C. AA range:401-450

特异性

AP-2γ Polyclonal Antibody detects endogenous levels of AP-2γ protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

预测分子量

45kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

transcription factor AP-2 gamma(TFAP2C) Homo sapiens The protein encoded by this gene is a sequence-specific DNA-binding transcription factor involved in the activation of several developmental genes. The encoded protein can act as either a homodimer or heterodimer with other family members and is induced during retinoic acid-mediated differentiation. It plays a role in the development of the eyes, face, body wall, limbs, and neural tube. [provided by RefSeq, Jul 2008],

组织表达

Liver,Mammary tumor,Ovary,Skin,

细胞定位

Nucleus .

功能

domain:The WW-binding motif mediates interaction with WWOX.,function:Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.,induction:During retinoic acid-mediated differentiation.,online information:Activatin protein 2 entry,PTM:Sumoylated on Lys-10; which inhibits transcriptional activity.,similarity:Belongs to the AP-2 family.,subunit:Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members (By similarity). Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HepG2 and HUVEC cells, using AP2C Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from K562 cells using AP2C antibody.

Western Blot analysis of various cells using AP-2γ Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.