BD-PT0251-AP-1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AP-1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0251

产品名称

AP-1 Rabbit Polyclonal Antibody

别名

JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39

类别

常规抗体

基因名称

JUN

蛋白名称

Transcription factor AP-1;jun;c-jun；AP-1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3725

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3725

Human Swissprot No.

P05412

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P05412/entry

Mouse Gene ID

16476

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16476

Mouse Swissprot No.

P05627

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P05627

Rat Gene ID

24516

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24516

Rat Swissprot No.

P17325

Rat Swissprot Link

http://www.uniprot.org/uniprot/P17325

免疫原

The antiserum was produced against synthesized peptide derived from human c-Jun. AA range:58-107

特异性

AP-1 Polyclonal Antibody detects endogenous levels of AP-1 protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

39-42kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008],

组织表达

Expressed in the developing and adult prostate and prostate cancer cells.

细胞定位

Nucleus.

信号通路

MAPK_ERK_Growth;MAPK_G_Protein;ErbB_HER;WNT;WNT-T CELLFocal adhesion;Toll_Like;T_Cell_Receptor;B_Cell_Antigen;Neurotrophin;GnRH;Epithelial cell signaling in Helicobacter pylori infection;Pathways in cancer;Colorectal cancer;Renal cell carcinoma;

功能

function:Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.,PTM:Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Jun subfamily.,similarity:Contains 1 bZIP domain.,subunit:Heterodimer with either FOS or BATF3. Interacts with HIVEP3 (By similarity). Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using c-Jun Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, using c-Jun Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of human-lung tissue. 1,AP-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-stomach tissue. 1,AP-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-lung tissue. 1,AP-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of various cells using primary antibody diluted at 1:1000（4°C overnight）. Secondary antibody：Goat Anti-rabbit IgG IRDye 800（ diluted at 1:5000, 25°C, 1 hour）. Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit（SM-005, Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,AP-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,AP-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using AP-1 Polyclonal Antibody diluted at 1：2000

Western Blot analysis of KB cells using AP-1 Polyclonal Antibody diluted at 1：2000